Guidelines for monitoring autophagy in Caenorhabditis elegans

分类： 生物学 >> 生物物理学 >> 细胞生物学 提交时间： 2016-05-12

Zhang, Hong Guo, Bin Lin, Long Lu, Qun Wu, Fan Chang, Jessica T. Hansen, Malene Kumsta, Caroline Lapierre, Louis R. Jia, Kailiang Kovacs, Attila L. Legouis, Renaud Melendez, Alicia Melendez, Alicia O'Rourke, Eyleen J. Sato, Ken Sato, Miyuki Wang, Xiaochen

摘要： The cellular recycling process of autophagy has been extensively characterized with standard assays in yeast and mammalian cell lines. In multicellular organisms, numerous external and internal factors differentially affect autophagy activity in specific cell types throughout the stages of organismal ontogeny, adding complexity to the analysis of autophagy in these metazoans. Here we summarize currently available assays for monitoring the autophagic process in the nematode C. elegans. A combination of measuring levels of the lipidated Atg8 ortholog LGG-1, degradation of well-characterized autophagic substrates such as germline P granule components and the SQSTM1/p62 ortholog SQST-1, expression of autophagic genes and electron microscopy analysis of autophagic structures are presently the most informative, yet steady-state, approaches available to assess autophagy levels in C. elegans. We also review how altered autophagy activity affects a variety of biological processes in C. elegans such as L1 survival under starvation conditions, dauer formation, aging, and cell death, as well as neuronal cell specification. Taken together, C. elegans is emerging as a powerful model organism to monitor autophagy while evaluating important physiological roles for autophagy in key developmental events as well as during adulthood.

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